Table 2.
Relative changes in protein expression between S. cerevisiae wild type and engineered strains
| Protein | Description | No. of peptides | Average of B/A | Average of C/A | Average of D/A |
|---|---|---|---|---|---|
| Galactose metabolism | |||||
| GAL1 | Galactokinase | 9 | 0.870 ± 0.340 | 0.587 ± 0.225 | 3.783 ± 0.215 |
| GAL7 | Galactose-1-phosphate uridylyltransferase | 2 | 1.230 ± 0.005 | 1.387 ± 0.965 | 1.008 ± 0.021 |
| Glycolysis | |||||
| HXK1 | Hexokinase-1 | 2 | 0.723 ± 0.259 | 0.691 ± 0.104 | 3.489 ± 0.368 |
| PGI1 | Glucose-6-phosphate isomerase | 3 | 1.032 ± 0.460 | 1.330 ± 0.180 | 9.891 ± 0.251 |
| PFK2 | Phosphofructokinase | 3 | 1.236 ± 0.155 | 1.149 ± 0.100 | 1.426 ± 0.197 |
| FBA1 | Fructose-biophosphate aldolase | 8 | 1.094 ± 0.155 | 0.871 ± 0.235 | 2.304 ± 0.942 |
| TPI1 | Triosephosphate isomerase | 6 | 1.123 ± 0.380 | 0.849 ± 0.070 | 1.038 ± 0.357 |
| TDH | Glyceraldehyde 3-phosphate dehydrogenase | 15 | 1.117 ± 0.305 | 0.925 ± 0.220 | 1.664 ± 0.541 |
| PGK1 | Phosphoglycerate kinase | 19 | 1.066 ± 0.225 | 0.798 ± 0.120 | 1.286 ± 0.076 |
| GPM1 | Phosphoglycerate mutase 1 | 14 | 1.047 ± 0.210 | 0.750 ± 0.145 | 1.500 ± 0.457 |
| ENO | Enolase | 19 | 1.185 ± 0.160 | 0.912 ± 0.155 | 2.176 ± 0.478 |
| PYK1 | Pyruvate kinase | 6 | 1.186 ± 0.200 | 0.831 ± 0.195 | 1.831 ± 0.147 |
| TCA cycle | |||||
| CIT1 | Citrate synthase, mitochondrial | 3 | 1.256 ± 0.820 | 1.982 ± 0.230 | 5.580 ± 0.248 |
| ACO1 | Aconitate hydratase, mitochondrial | 3 | 0.961 ± 0.400 | 1.033 ± 0.075 | 1.737 ± 0.128 |
| ATP synthesis | |||||
| ATP1 | ATP synthase subunit alpha, mitochondrial | 6 | 1.222 ± 0.260 | 1.005 ± 0.030 | 1.157 ± 0.160 |
| ATP2 | ATP synthase subunit beta, mitochondrial | 8 | 1.176 ± 0.040 | 1.123 ± 0.085 | 3.216 ± 0.205 |
| Amino-acid metabolism | |||||
| LEU1 | 3-isopropylmalate dehydratase | 3 | 0.883 ± 0.075 | 1.023 ± 0.335 | 1.424 ± 0.200 |
| LEU2 | 3-isopropylmalate dehydrogenase | 17 | 3.477 ± 0.630 | 1.070 ± 0.335 | 2.570 ± 0.254 |
| MET6 | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase | 10 | 1.050 ± 0.125 | 0.881 ± 0.430 | 2.018 ± 0.121 |
| PDC | Pyruvate decarboxylase isozyme | 12 | 1.305 ± 0.355 | 1.118 ± 0.115 | 1.894 ± 0.218 |
| Protein biosynthesis | |||||
| TIF | ATP-dependent RNA helicase eIF4A | 3 | 1.408 ± 0.025 | 0.752 ± 0.295 | 1.655 ± 0.245 |
| TEF1 | Elongation factor 1-alpha | 8 | 0.910 ± 0.375 | 0.758 ± 0.185 | 1.507 ± 0.110 |
| RPL4 | 60s ribosomal protein L4 | 9 | 1.245 ± 0.255 | 0.778 ± 0.035 | 1.418 ± 0.068 |
| RPL19 | 60s ribosomal protein L19 | 2 | 1.218 ± 0.285 | 1.114 ± 0.805 | 2.995 ± 0.197 |
| Heat shock proteins | |||||
| HSP 12 | 12 kDa Heat shock protein | 2 | 2.199 ± 0.640 | 0.882 ± 0.135 | 2.308 ± 0.219 |
| HSP 26 | Heat shock protein 26 | 3 | 2.281 ± 0.675 | 1.823 ± 0.360 | 2.453 ± 0.195 |
| STI1 | Heat shock protein STI1 | 2 | 1.363 ± 0.665 | 0.485 ± 0.035 | 3.450 ± 0.377 |
| Unknown | |||||
| POR1 | Mitochondrial outer membrane protein porin 1 | 4 | 1.033 ± 0.395 | 0.808 ± 0.445 | 2.785 ± 0.066 |
| SAM2 | S-adenosylmethionine synthetase 2 | 2 | 1.271 ± 0.300 | 0.624 ± 0.085 | 2.125 ± 0.151 |
| YMR226C | Uncharacterized oxidoreductase YMR226C | 2 | 1.856 ± 0.375 | 1.260 ± 0.110 | 3.051 ± 0.265 |
| SOD1 | Superoxide dismutase [Cu-Zn] | 2 | 6.360 ± 0.420 | 3.942 ± 1.400 | 7.910 ± 0.330 |
Average of protein expression levels in WT-9LHP strain was taken as 1 and the deviation was calculated from three independent LC-MS/MS analysis results. The “Average of B/A” refers to the average ratio of protein expression level in Δpxa1-9LHP strain over that in WT-9LHP strain. “Average of C/A”refers to the average ratio of protein expression level in Δpxa2-9LHP strain over that in WT-9LHP strain. The “Average of D/A” refers to the average ratio of protein expression level in Δpxa1&2-9LHP strain over that in WT-9LHP strain.