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. Author manuscript; available in PMC: 2016 May 27.
Published in final edited form as: Cell Rep. 2016 Mar 10;14(11):2528–2537. doi: 10.1016/j.celrep.2016.02.049

Figure 3. USP7 occupies p53 target promoters and maintains the H3K9me3 mark on these promoters.

Figure 3

a) ChIP analysis showing USP7 occupancy on p53 target promoters in HCT116 wt cells stably transduced with inducible shGFP or shUSP7 (two sequences) and cultured in the presence of doxycycline for 48h. b) Western blot analysis of HCT116 p53 wt and p53−/− cells treated with 0, 2.5, 5 or 10µM of HBX41108 for 24h. c) ChIP analysis showing H3K9me3 enrichment on p53 target promoters in HCT116 p53 wt cells stably transduced with inducible shGFP or shUSP7 (two sequences) and cultured in the presence of doxycycline for 48h. d, e) ChIP analysis showing USP7 occupancy (d) and H3K9me3 enrichment (e) in HCT116 p53 wt cells treated with 10µM of HBX41108 for 24h. See also Figures S4, S5 and S6.