FIGURE 2:

Staufen1 is recruited to SGs in Myoblasts. (A) Proliferative C2C12 myoblasts were untreated or treated with 0.5 mM arsenite for 45 min. Coimmunofluorescence staining was performed using Staufen1 and TIA-1 antibodies. (B) Proliferative C2C12 myoblasts were treated with CHX (50 μg/ml) or Puro (10 μg/ml) for the entire duration of the arsenite treatment and processed as in A. DAPI was used to stain nuclei. Arrowheads show SGs in myoblasts. Scale bars, 20 μm. For r, 18 cells were analyzed.