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Arsenite induces formation of SGs in myotubes. (A, B) Three-day-differentiated myotubes were untreated or treated with 0.5 mM arsenite for 45 min. Coimmunofluorescence stainings were performed using TIA-1 and DDX3 or TIA-1 and Staufen1 antibodies. (C) C2C12 myoblasts transfected with TIA-1-mCherry and Staufen1-GFP were differentiated for 3 d. DAPI was used to stain nuclei. Arrowheads show SGs in myoblasts. Scale bars, 20 μm. For r, 9, 9, and 12 cells were analyzed, respectively.
