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. 2016 Jun 1;27(11):1740–1752. doi: 10.1091/mbc.E15-09-0655

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© 2016 Zheng, Mennella, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Nin localizes to noncentrosomal MTOCs in wing epithelia and myocytes. (A) Nin-GFP associates with the noncentrosomal MTOC in wing epithelia. In the columnar epithelial cells of the developing wing disk, Nin-GFP (green) localizes primarily to one focus in each cell. The focus of Nin-GFP colocalizes adjacent to centrosomes ∼20% (29/156) of the time (top inset), and is unassociated in ∼80% (127/156) of cells (yellow arrows in insets). Centrosomes labeled with antibodies against asterless (asl), red). Dlg (purple) is an apical membrane marker. Image is an xy view of a third instar larval wing pouch epithelium z-stack projection. (B) Images of Nin-GFP foci in xy and xz views of the wing disk. These views demonstrate that Nin-GFP foci and centrosomes are both localized near the apical membrane in wing epithelia (yellow arrows). (C) Nin-GFP (green) localizes to the center of the noncentrosomal MTOCs labeled with α-tubulin (purple). (D) Myocytes in third instar larval muscles stained for endogenous Nin (green) using the C-terminal Nin antibody and DAPI (red) show that Nin has perinuclear localization. Yellow arrows point to the Nin localization at the periphery of myocyte nuclei. Scale bars: 5 μm in A–C, 1 μm in D.