FIGURE 1:

alp16Δ is synthetic lethal with the mzt1-T27W mutant, and Alp16^GCP6 localizes to mitotic SPBs in the absence of Gfh1^GCP4 and Mod21^GCP5. (A) alp16Δ is synthetic lethal with the mzt1-T27W mutant. Diploid cells obtained from crosses between mzt1-T27W-hphR and gfh1Δ-kanR, mod21Δ-kanR, or alp16Δ-kanR were sporulated, and individual spores (a–d) in each ascus (1–5) were dissected on YES plates. Nonviable spores from the cross between mzt1-T27W and alp16Δ germinated, divided several times, and arrested with elongated morphologies. Drug resistance of viable spores (orange circle) and predicted resistance of nonviable spores (white circle): k, kanR (resistance to G418); h, hphR (resistance to hygromycin B). (B) Localization of Alp16^GCP6, Gfh1^GCP4, and Mod21^GCP5 to mitotic SPBs. Localization of GFP-Alp16^GCP6, GFP-Gfh1^GCP4, and GFP-Mod21^GCP5 to mitotic SPBs was examined in wild-type (WT), gfh1Δ, mod21Δ, and alp16Δ cells containing mCh-Atb2 and Sid4-mRFP. Scale bar, 10 μm.