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. 2016 Jun 1;27(11):1753–1763. doi: 10.1091/mbc.E15-08-0577

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© 2016 Masuda and Toda. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Alp16^GCP6 overproduction suppresses the temperature sensitivity of mzt1 mutants but not the lethality of mzt1-null. (A) The temperature sensitivity of mzt1-T27W is rescued by Alp16^GCP6 overproduction. Serial dilution spot assays of wild-type, mzt1-T27W, mzt1-T27W lys1⁺-P3nmt-alp16, and mzt1-T27W lys1⁺-P41nmt-alp16 cells. Cells were spotted onto YES plates and incubated at various temperatures for 3 d. (B) Mzt1 is essential for growth in Alp16^GCP6-overproducing cells. Diploid cells homologous for lys1⁺-P3nmt-alp16 and heterozygous for mzt1 (mzt1⁺/mzt1Δ::natR) were sporulated, and individual spores (a–d) in each ascus (1–4) were dissected on YES to partially repress the expression of Alp16^GCP6 and on PMG plates to highly induce the expression. Only mzt1⁺ segregants sensitive to clonNAT grew in both conditions.