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. 2016 Jun 1;27(11):1834–1844. doi: 10.1091/mbc.E15-03-0123

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© 2016 Cox et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Differential effect of GFP-Rab43 on G^AE and G sorting. COS7 cells transfected with GFP-Rab43 were subsequently infected with replication-defective adenoviruses encoding (A) G^AE -Ds or (B) G-Ds and grown at the restrictive temperature for 24 h. The cells were then shifted to permissive temperature for 30 min, fixed, and imaged by confocal microscopy. The merged images show that G^AE accumulated in a GFP-Rab43-positive compartment, whereas G did not. (C) Percentage colocalization of G^AE and G with GFP-Rab43. The values represent the average (±SD) from at least 20 cells from two independent experiments. **p < 0.0001 (Student’s two-tailed t test). Scale bars, 5 μm.