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. 2016 Jun 1;27(11):1845–1852. doi: 10.1091/mbc.E16-03-0154

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© 2016 Johnson and Pfeffer. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Effect of ezetimibe addition on NPC1L1 endocytosis. Cells were pretreated for 60 min with 30 μM ezetimibe; endocytosis was carried out in the continued presence of ezetimibe. (A) Representative gel in the presence of ezetimibe as in Figure 2B. (B) Combined results of three experiments with each point assayed in duplicate. Control values are from the experiments presented in Figure 3. Error bars represent SEM.