FIGURE 6:

(A) Ezetimibe-sensitive cholesterol uptake in cells expressing NPC1L1 protein. Parental RH7777 cells (right) or RH7777 cells expressing human NPC1L1-GFP (left) were monitored for [³H]cholesterol uptake from sodium taurocholate micelles ± 30 μM ezetimibe for 20 min as described in Materials and Methods; 39 μg of each sample was counted. The average of two experiments carried out in quadruplicate. ****p < 0.0001 for ± ezetimibe for NPC1L1 cells (unpaired t test). (B) Cholesterol uptake in RH7777 cells expressing NPC1L1-WT or NPC1L1-ΔC proteins; 19 μg of each sample was counted. A representative experiment carried out in quadruplicate. Error bars represent SEM.