(
A) Cell cycle composition as measured by propidium iodide staining of DNA and flow cytometry analysis of cells that have been depleted of CLK1 by the indicated shRNAs. (
B) Representative histograms of propidium iodide stained H157cells to determine cell cycle defect after RNAi of CLK1. (
C) HeLa cells treated with TG003 also have defective cell division. Immunofluorescence microscopy was used to detect multi-nucleated cells, and a representative field is shown (green:tubulin, red:emerin, blue:DAPI). (
D) Representative histograms of DNA content in synchronous HeLa cells treated with TG003. The time after early S phase release (when TG003 was added) is indicated, These data are associated with
Figure 4 of the main text. (
E) Representative image from anchorage-independent growth assays (soft agar assay) of HeLa cells after depletion of CLK1. (
F) Relative mRNA expression levels of
CENPE and
HMMR during cell cycle. Data obtained from RNA-Seq analysis. Dashed line represents 6 hr after release from G1/S.