(
A) Subsets of KC types labelled in GAL4 lines and the resulting learning defects in the behavioral screen using
UAS-shits1. Expression levels (represented in the gray scale) are determined as previously described (
Paulk and Gronenberg, 2008);
† stochastic expression. Stars in the left column indicate the statistical significance of memory impairment compared to the
UAS-shi/+ control (Dunn’s multiple pairwise comparison);
n = 8–119. (
B) Expression pattern of
MB419B-GAL4 which specifically labels γd KCs. Scale bar represents 50 µm. Grey background staining: Discs large. (
C) Blocking output of the γd neurons with
MB419B-GAL4 (
B; one-way ANOVA,
post-hoc pairwise comparison, p<0.05;
n = 18–60) impairs aversive color discrimination learning. (
D) The same blockade does not significantly affect aversive olfactory conditioning (one-way ANOVA, p>0.05;
n = 8–10).