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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: J Endod. 2016 Apr 27;42(6):928–934. doi: 10.1016/j.joen.2016.03.009

Figure 3.

Figure 3

CLSM analysis of live/dead bacteria in dentinal tubules. (A) Positive and negative control sample images. Red indicates dead bacteria (−Ve control), and green indicates live bacteria (+Ve control) in dentinal tubules. Negative control was inoculated as for experimental samples and then autoclaved; positive control was inoculated but not disinfected. (B) Representative stacked images from coronal, middle, and apical thirds of the root showing dentinal tubules in samples treated by different irrigation methods: SNI, EA, XP Endo, and PIPS. C, canal side; D, dentin.