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. 2016 May 25;20(9):1611–1619. doi: 10.1111/jcmm.12852

Figure 4.

Figure 4

Rac1 is a direct target of miR‐224. (A) The 3′UTR of Rac1 contains one predicted miR‐224 binding site. The mutagenesis nucleotides are indicated in red. (B) Overexpression of miR‐224 suppressed Rac1 mRNA expression in MG‐63 cells. (C) Overexpression of miR‐224 suppressed Rac1 protein expression in MG‐63 cells. (D) MG‐63 cells were cotransfected with 3′UTR‐reporter constructs and a miR‐224 mimic or a scramble sequence, and dual luciferase reporter assays were performed. (E) Rac1 expression was measured in osteosarcoma cell lines (MG‐63, U2OS, SOSP‐9607 and SAOS‐2) and hFOB cells by qRT‐PCR. **P < 0.01 and ***P < 0.001.