Figure 1.

Kiss1, vGluT2, and vGAT expression in Kiss1^ARC neurons. A, qPCR assay with amplification curves for Kiss1, vGluT2, and vGAT (all analyzed in 5-cell pools). Cycle number of castrated males was plotted against the normalized fluorescence intensity (ΔRn) to visualize the PCR amplification. The cycle threshold (dashed line) is the point in the amplification at which sample values were calculated. Melting curves depict single-product melting at 82.8°C and 83.7°C for vGluT2 and vGAT, respectively, illustrating that only 1 product was formed from each transcript in the Kiss1^ARC neuronal pools. B, Bar graph summarizing mRNA expression of Kiss1, Slc17a6 (vGluT2), and Slc32a1 (vGAT) from pooled Kiss1^Cre:GFP neurons relative to the calibrator (see Materials and Methods) of gonad-intact (INT) and castrated (CX) male mice. Animal numbers are indicated. Using Newman-Keuls multiple comparison test; **, P < .01; ***, P < .001. Slc32a1 mRNA in pooled Kiss1^ARC neurons was not detectable (ND).