Skip to main content
. 2016 Apr 19;30(6):630–644. doi: 10.1210/me.2016-1026

Figure 2.

Figure 2.

ARC YFP cells contain mRNA for Kiss1 and vGluT2. A, Photomicrograph of the ARC 14 days after bilateral ARC injection of AAV1-EF1α-DIO-ChR2:YFP in a Kiss1Cre:GFP castrated male mouse at a low-power magnification (scale bar, 200 μm). 3V, third ventricle; ME, median eminence. B, Photomicrograph of the periventricular nucleus illustrating YFP fibers (scale bar, 20 μm). 3V, third ventricle. C, Representative gels illustrating mRNA expression of Kiss1 and Slc17a6 (vGluT2) in single YFP cells. The expected base pair (bp) sizes for Kiss1 and Slc17a6 are 120 and 136 bp, respectively. Exclusion of reverse transcriptase (−RT) in a reacted cell was used as a negative control. RNA extracted from the medial basal hypothalamic tissue was also included as positive (+, with RT) and negative (−, without RT) tissue controls. MM, molecular marker. D, Bar graph summarizing the percentage (mean ± SEM) of YFP cells that expressed mRNAs that encode for Kiss1, POMC, AgRP, vGluT2, and vGAT. Note: only 1 out of 30 cells expressed mRNA for vGAT.