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EM revealed vGluT2-immunoreactivity in Kiss1ARC axon terminals. A, A dual-labeled ARC Kiss1-ChR2:YFP axon terminal (t) containing both vGluT2 (immunoperoxidase) and YFP (immunogold particles) and forms an asymmetrical synapse with a postsynaptic density (curved arrow) onto an unlabeled ARC dendrite (d). Scale bar, 500 nm. B, Schematic representation of A with a Kiss1ARC terminal (containing vGluT2 and YFP) forming an asymmetrical (glutamatergic) synapse with an unlabeled ARC dendrite.
