Figure 7.

Steroid-dependent change in glutamate release from Kiss1^ARC neurons. Whole-cell, voltage-clamp recording (V_h = −60 mV) of nonfluorescent AgRP neurons (post hoc identified) using a photo-stimulation paired-pulse paradigm (pulse duration, 5 ms; interpulse interval, 50 ms) in male Kiss1^Cre:GFP::Ai32 mice and AAV-EF1α-DIO-ChR2:YFP injected Kiss1^Cre:GFP mice. A representative trace that averages 10 sequential paired-pulse recordings is shown for a gonad-intact (A) and castrated (B) male Kiss1^Cre:GFP::Ai32 mouse. C, Bar graph summarizes the averaged (±SEM) PPR (pulse 2 amplitude to pulse 1 amplitude) of the induced EPSC from gonad-intact and castrated male Kiss1^Cre:GFP::Ai32 and AAV injected Kiss1^Cre:GFP mice. Cell numbers are indicated. Using an unpaired Student's t test; **, P < .01.