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. 2016 Apr 19;30(6):630–644. doi: 10.1210/me.2016-1026

Figure 8.

Figure 8.

Glutamate differentially regulates POMC and AgRP neurons via mGluR signaling. A, Whole-cell current clamp recording of a PomcEGFP neuron in the presence of CNQX (10μM) and AP5 (50μM) with the fast perfusion of glutamate (100μM). Whole-cell current clamp recording of a PomcEGFP neuron with (B) DHPG (50μM) alone or (C) DHPG in the presence of CNQX (10μM), AP5 (50μM), and TTX (1μM). D, Bar graph summarizing the effect of DHPG alone, DHPG with blockers (CNQX, AP5, and TTX), and DCG-IV alone on POMC neurons. Cell numbers are indicated. E, Whole-cell current clamp recording of an ARC NpyGFP neuron in the presence of CNQX (10μM) and AP5 (50μM) with the fast perfusion of glutamate (100μM). Whole-cell current clamp recording of an ARC NpyGFP neuron with (F) DCG-IV (10μM) alone or (G) DCG-IV (10μM) in the presence of CNQX (10μM), AP5 (50μM), TTX (1μM), and picrotoxin (100μM). H, Bar graph summarizing the effect of DCG-IV alone, DCG-IV with blockers (CNQX, AP5, and TTX), and DHPG alone on AgRP neurons. Cell numbers are indicated. Using Newman-Keuls multiple comparison test; *, P < .05; **, P < .01.