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. 2016 Apr 19;30(6):630–644. doi: 10.1210/me.2016-1026

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Copyright © 2016 by the Endocrine Society

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Figure 9. — High frequency photo-stimulation of ChR2 Kiss1^ARC neurons stimulates POMC neurons and inhibits AgRP neurons. A, Representative confocal image of the ARC from a Kiss1^Cre:GFP::Pomc^EGFP male mouse injected with AAV1-EF1α-DIO-ChR2:mCherry. Scale bar, 50 μm. 3V, third ventricle. B, High frequency photo-stimulation of ChR2 (20 Hz, solid blue bar) evoked a slow depolarization (EPSP) in a Pomc^EGFP neuron. C, Bar graph summarizing the effect of 20-Hz photo-stimulation in POMC neurons (n = 7). D, Representative confocal image of the ARC from a Kiss1^Cre:GFP::Npy^GFP male mouse injected with AAV1-EF1α-DIO-ChR2:mCherry. Scale bar, 50 μm. 3V, third ventricle. E, High frequency photo-stimulation of ChR2 (20 Hz, solid blue bar) evoked a slow hyperpolarization (IPSP) in an ARC Npy^GFP neuron. F, Bar graph summarizing the effect of 20 Hz LED stimulation in AgRP neurons (n = 5). Note the hyperpolarization typical for activation of Gα_i.o-coupled receptors.