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. 2016 Apr 19;30(6):630–644. doi: 10.1210/me.2016-1026

Table 1.

Primer Sequences and Annealing Temperatures Used for scRT-PCR and qPCR

Gene Name (Encodes for)	Accession Number	Forward Primer (5′-3′) Reverse Primer (5′-3′)	Product Size (bp)	Annealing Temperature (°C)
Kiss1 (Kiss1)^a	NM_178260	`TGCTGCTTCTCCTCTGT`	120	57
		`ACCGCGATTCCTTTTCC`
Pomc (POMC)^a	NM_008895	`GGAAGATGCCGAGATTCTGC`	200	60.5
		`TCCGTTGCCAGGAAACAC`
Npy (NPY)^a	NM_023456	`ACTGACCCTCGCTCTATCTC`	182	60
		`TCTCAGGGCTGGATCTCTTG`
Agrp (AgRP)^a	NM_001271806	`CTCCACTGAAGGGCATCAGAAG`	146	59
		`ACTCAGCACCTCCGCCAAAG`
Slc17a6 (vGluT2)^a	NM_080853	`ATCTGCTAGGTGCAATGG`	136	57
		`TAAGCTGGCTGACTGATG`
Slc17a6 (vGluT2)^b	NM_080853	`TCCCTCGGACAGATCTAC`	113	60
		`CATAGCGGAGCCTTCTTC`
Slc32a1 (vGAT)^ab	NM_009508	`GTCACGACAAACCCAAGATCAC`	137	59^a, 60^b
		`GGCGAAGATGATGAGGAACAAC`
Actb (β-actin)^a	NM_007393	`AAGGCCAACCGTGAAAAGAT`	110	60
		`GTGGTACGACCAGAGGCATAC`
Gapdh (GAPDH)^b	NM_008084	`GCACCACCAACTGCTTAG`	93	60
		`CAGTGATGGCATGGACTG`

^a

Primers used for scRT-PCR.

^b

Primers used for qPCR.