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. 2016 May 27;17:412. doi: 10.1186/s12864-016-2747-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Fluorescence activated cell sorting (FACS) analysis of stained cells and validation of cell cycle sorted populations in HDFa, NCI-H295R and HeLa cells. Panels a-c: FACS analysis shows cell cycle distribution of stained cells before (first image) and after sorting to G1, S and G2 phases (second, third and fourth images, respectively; Panel a: HDFa, Panel b: NCI-H295R, Panel c: HeLa. Intervals of fluorescence intensity (shown as vertical bands) were defined to gate G1, S and G2 phases, respectively. Panels d-e: Western blot analysis confirms the high efficacy of cell cycle sort (Panel d: NCI-H295R, Panel e: HeLa). Density values of phospho(Tyr15)-CDC-2 were first normalized to β-actin loading control and were further normalized to G1 phase (displayed above each band)