Fig. 7.

Tissue-specific expression patterns of TaAnn genes. a Tissue-specific expression patterns of 15 TaAnn genes detected in the microarray data (GSE12508). The heat map was generated by hierarchical clustering based on Pearson’s correlation, and log2 ratios of expression were used to make the map. b Expression levels of TaAnn8, TaAnn9 and TaAnn10 genes in wheat seedings and stamen before flowering. Total RNA extracted from the seed, 7-day and 14-day-old young root, stem, leaf tissues and stamen before flowering of wheat Jinghua 9 was used for real-time PCR. The expression in the seed sample was set as 1. c The expression pattern of TaAnn10 gene in the taiyuan806 recovery line. Total RNA extracted from the spike at stamen and pistil initiation stage, anther at anther separation stage, anther at meiosis stage, and spike not including anther at meiosis stage of wheat taiyuan806 was used for real-time PCR. 1 is spike at stamen and pistil initiation stage; 2 is anther at anther seperation stage; 3 is anther at meiosis stage; 4 is spike not including anther at meiosis stage. The expression at the spike not including anther at meiosis stage sample was set as 1. The expression level of wheat actin was used as the internal control to standardize the RNA samples for each reaction. The data are from three biological replicates, and error bars represent the standard error