Table 3.
Complementary expressions between miRNAs and their targets in flax under saline, alkaline, and saline-alkaline stresses
| MiRNAs | Small RNA sequencing | Target gene | Annotation | Degradome sequencing | DGE sequencing | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| AS | AS2 | NSS | Position | Lignment score | Category | AS | AS2 | NSS | |||
| lus-miR156g/a | Down | Down | Down | Lus10000257 | Tetratricopeptide repeat (TPR)-like superfamily protein | 761 | 4.5 | 4 | Up | Up | Up |
| lus-miR159b | Down | Down | Down | Lus10010495 | Cystatin/monellin superfamily protein | 335 | 4.5 | 3 | Up | Up | Up |
| lus-miR160a/b/d/e/f/h/i/j | Up | Up | Down | Lus10041268 | Transducin/WD40 repeat-like superfamily protein | 1014 | 4 | 2 | Down | Down | Up |
| lus-miR162a/b | Down | Down | Down | Lus10015483 | Heat shock protein 70 (Hsp 70) family protein | 1243 | 4.5 | 2 | Up | Up | Up |
| lus-miR164a/b/c/d/e | Down | Up | Down | Lus10006635 | ARM repeat superfamily protein | 153 | 4 | 4 | Up | Down | Up |
| lus-miR166a/c/d/g/h/j | Down | Down | Down | Lus10020493 | Pathogenesis-related gene 1 | 220 | 4 | 2 | Up | Up | Up |
| lus-miR167a | Down | Down | Down | Lus10014324 | G-box binding factor 1 | 679 | 3.5 | 2 | Up | Up | Up |
| lus-miR168a/b | Down | Down | Down | Lus10000074 | Methionine gamma-lyase | 469 | 4.5 | 4 | Up | Up | Up |
| lus-miR169a/d | Up | Down | Down | Lus10014674 | Transducin/WD40 repeat-like superfamily protein | 638 | 4 | 4 | Down | Up | Up |
| lus-miR169e/i | Down | Down | Down | Lus10006846 | Profilin 5 | 96 | 4.5 | 4 | Up | Up | Up |
| lus-miR169g/l | Up | Down | Up | Lus10030904 | Alpha/beta-Hydrolases superfamily protein | 755 | 4.5 | 4 | Down | Up | Down |
| lus-miR171b/c/e | Down | Down | Down | Lus10009876 | UDP-glucosyl transferase 85A3 | 952 | 4 | 4 | Up | Up | Up |
| lus-miR171d | Up | Down | Down | Lus10017991 | Jasmonate-zim-domain protein 3 | 594 | 4.5 | 2 | Down | Up | Up |
| lus-miR172a/b/c/d/f/h | Down | Down | Down | Lus10001322 | Deoxyxylulose-5-phosphate synthase | 1796 | 4 | 4 | Up | Up | Up |
| lus-miR319b | Down | Down | Down | Lus10009442 | O-methyltransferase family protein | 502 | 4 | 4 | Up | Up | Up |
| lus-miR390a/b/c/d | Down | Down | Down | Lus10015906 | Purine permease 3 | 857 | 4.5 | 4 | Up | Up | Up |
| lus-miR393a/b/c/d | Up | Up | Down | Lus10040438 | F-box family protein | 1620 | 3.5 | 4 | Down | Down | Up |
| lus-miR394a/b | Up | Up | Down | Lus10018337 | Pyruvate dehydrogenase kinase | 536 | 4.5 | 2 | Down | Down | Up |
| lus-miR395a/b/c/d | Down | Down | Down | Lus10006629 | ATP sulfurylase 1 | 339 | 2.5 | 0 | Up | Up | Up |
| lus-miR396a/c | Down | Down | Down | Lus10001321 | Xylose isomerase family protein | 648 | 3.5 | 2 | Up | Up | Up |
| lus-miR397a | Up | Up | Down | Lus10004434 | REF4-related 1 | 1875 | 4.5 | 4 | Down | Down | Up |
| lus-miR397b | Down | Up | Up | Lus10001002 | 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase | 581 | 4.5 | 4 | Up | Down | Down |
| lus-miR398a | Up | Up | Up | Lus10016155 | Copper/zinc superoxide dismutase 2 | 449 | 4 | 0 | Down | Down | Down |
| lus-miR398b/c/d/e | Down | Down | Down | Lus10003315 | Myosin family protein with Dil domain | 4406 | 4.5 | 2 | Up | Up | Up |
| lus-miR399b/d | Down | Up | Down | Lus10019360 | Trigalactosyldiacylglycerol2 | 323 | 4.5 | 4 | Up | Down | Up |
| lus-miR399f/g | Up | Up | Up | Lus10003060 | Cofactor-independent phosphoglycerate mutase | 1097 | 4 | 4 | Down | Down | Down |
| lus-miR408a | Up | Up | Up | Lus10003138 | Cyclophilin 20-2 | 860 | 4.5 | 2 | Down | Down | Down |
| lus-miR530a/b | Up | Up | Down | Lus10001902 | WRKY family transcription factor | 490 | 4.5 | 2 | Down | Down | Up |
| lus-miR828a | Down | Up | Up | Lus10013640 | Ribosomal protein L3 family protein | 570 | 4.5 | 4 | Up | Down | Down |
Abbreviations: ARM Armadillo, AS Alkaline stress, AS2 Alkaline-salt stress, ATP Adenosine-triphosphate, CK Control, DGE Digital gene expression, Hsp Heat shock protein, Lus Linum usitatissimum, MiRNA MicroRNA, NSS Neutral salt stress, REF Reduced epidermal fluorescenc, TPR Tetratricopeptide repeat, UDP Uridine diphosphate