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. Author manuscript; available in PMC: 2016 Sep 22.
Published in final edited form as: Lab Invest. 2016 Mar 14;96(6):623–631. doi: 10.1038/labinvest.2016.40

Figure 2. The effect of Slpi deletion on alveolar macrophage TGF-β activation and pulmonary fibrosis.

Figure 2

(a) pSmad2 levels in nuclear extracts from BAL cells from Slpi−/− and wild-type mice 28 days post-bleomycin (BLM). Data expressed as mean absorbance at 450 nm ± SEM; n ≥ 5; **P < 0.005 BLM vs. saline. (b) pSmad2 immunohistochemistry. Representative images captured using original magnification x20, scale bars 100 μm. Arrowheads identify positively stained cells within a single, representative, alveolus in saline treated mice only; there is just one arrowhead in the wild-type mouse and three in the Slpi−/− mouse. (c) Masson’s trichrome staining 28 days post-BLM treatment. Representative, whole lung lobe, cross sections. (d) Quantitative assessment of trichrome staining. Data expressed as mean Ashcroft Score ± SEM; n ≥ 3.