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. Author manuscript; available in PMC: 2016 Sep 22.
Published in final edited form as: Lab Invest. 2016 Mar 14;96(6):623–631. doi: 10.1038/labinvest.2016.40

Figure 4. The effect of Slpi deletion on subtype specific collagen synthesis and deposition.

Figure 4

(a) Col1α1, Col3α1, Col4α1 and Col6α1 gene expression 0, 7, 14 and 28 days post-bleomycin (BLM). Data expressed as mean relative expression (ΔΔCt) ± SEM; n ≥ 8, *P < 0.05, ** P < 0.01, ***P < 0.001 and **** P < 0.0001. (b) 5 μm thick tissue sections from BLM treated wild-type and Slpi−/− mice were stained with anti-collagen I, anti-collagen III, anti-collagen IV and anti-collagen VI. Low magnification (x10) images were stitched together to visualise whole lung lobes. Images are representative of n ≥ 3 animals. (c) Collagen I and VI deposition was measured in lung tissue sections. Data expressed as mean percentage of total lung tissue comprised of fibrotic lesions greater than 7 500 μm2 containing collagen I and collagen VI (%) ± SEM; n ≥ 3.