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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Virology. 2016 Apr 8;494:23–28. doi: 10.1016/j.virol.2016.04.002

Figure 3.

Figure 3

Virus binding in the absence of heparan sulfate proteoglycans or in the presence of an inhibitory ligand for heparan sulfate. (A) gH-null virus was incubated for 1h on ice with CHO K1, CHO pgs-745 or CHO pgsD-677 cells as indicated, cells were washed and virus (BamK) and cell (18s rRNA) DNA were determined by QPCR. Binding is expressed as a percent of binding to CHO K1. The experiment was repeated 3 times and the difference between binding to CHO K1 versus the other two CHO mutant lines has a p value of <0.001 as calculated by an unpaired T-test. (B) CHO K1 cells were incubated with indicated concentrations of DSTP 27 for 30 min and then gH-null virus was added for 1h on ice. Cells were washed and virus (BamK) and cell (18s rRNA) DNA were measured by QPCR.