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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Virology. 2016 Apr 8;494:23–28. doi: 10.1016/j.virol.2016.04.002

Figure 5.

Figure 5

Only the aminoterminal two short consensus repeats are needed to enhance infection. AGS cells were nucleoporated with empty vector, plasmids expressing full length CD21, CD21 lacking a cytoplasmic tail (CD21-CT), CD21 comprised of only the aminoterminal 2 SCRs (2SCR) or the aminoterminal 2SCRs anchored by the cytoplasmic tail and transmembrane domain of gH (2SCR-gH). At 24 h cells were stained with an antibody to the aminoterminal 2SCRs and examined by flow cytometry (black bar), the number of gH-null virus copies able to bind was measured by QPCR (hatched bar), and cells were infected with virus expressing GFP and measured for GFP expression by flow cytometry 48 h later (grey bar). Values are expressed as a percent of those achieved with full length CD21. The experiment was repeated with the same results.