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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Neurotoxicol Teratol. 2016 Mar 26;55:23–31. doi: 10.1016/j.ntt.2016.03.002

Figure 2.

Figure 2

Figure 2

Decreased neuron-associated green fluorescence following chronic TD exposure. Following treatment of eggs from the pan-neuronal::GFP (NW1229) strain with varying concentrations of TD (as percent glyphosate), number of green pixels was determined (see Section 2.5). Data from analyzing pixel number associated with either the entire worm (a) or only the ventral nerve cord (b) are presented as mean pixel number ± SD. *p < 0.05, ***p < 0.001 compared to CN. Photomicrographs of a representative control (c) or treated (d) worm at the L4 stage show abnormal regions in the nervous system. The photomicrograph of the CN worm demonstrates nerve ring neurons in close proximity to the cuticle, and nerve cord neurons with their associated neurites. The representative photomicrograph of an age-matched treated worm shows gaps or diminished connectivity of neurites (blue arrows) and apparent morphological changes in nerve cord neurons (yellow arrow).