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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Trends Parasitol. 2016 Apr 6;32(6):446–457. doi: 10.1016/j.pt.2016.03.005

Figure 5. Genes encoding translation machinery in Trypanosoma brucei show stage-regulated protein production.

Figure 5

Clustering analysis groups genes with similar expression patterns, in this case for ribosome footprints (protein production) and mRNA abundance. The 1557 genes showing at least a four-fold difference in ribosome footprint normalized read counts were clustered by K-Means and HCL using MEV (for details see [12]). Biological samples included three replicates of PCF, animal-derived slender BF (slBF), and cultured BF (cBF). Each gene is represented by a separate line in the cluster diagram with the magnitude of the fold-change (relative to PCF median) indicated by color intensity (magenta for up and green for down). Bars at right indicate genes within each cluster that can be classified into the listed functional groups. Cluster A3 is highly enriched for proteins involved in translation, particularly structural components of the cytosolic ribosome. Reproduced from [12].