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. Author manuscript; available in PMC: 2017 Jun 5.
Published in final edited form as: J Mol Biol. 2016 Apr 2;428(11):2259–2264. doi: 10.1016/j.jmb.2016.03.027

Fig. 4. Gram-positive Hfq proteins do not recognize A-rich target RNA.

Fig. 4

(a) Binding of FAM-labeled rA18 RNA to the distal face of Hfq was measured by fluorescence anisotropy, as previously described (15). (b) Fractional binding versus [Hfq6] was measured and fitted to a two-state Hill equation as described in Fig. 3b.