Skip to main content
. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: DNA Repair (Amst). 2016 Apr 21;42:82–93. doi: 10.1016/j.dnarep.2016.04.006

Fig. 7.

Fig. 7

RNF138 mediates degradation of RAD51D through the ubiquitin-proteasome pathway. (A) Analysis of RNF138 facilitated RAD51D ubiquitination in vivo. (B) Rad51d−/− Trp53−/− HARad51d MEFs were transfected with Myc-RNF138 (upper panels) or 30nM Rnf138 siRNA1 (lower panels). HA-RAD51D protein levels were assessed 2, 4, and 6 hours following initiation of CHX block. HA-RAD51D band intensity was normalized to β-actin and plotted as percent protein remaining for each time point. Data represent mean +/− STD from two representative experiments. (C) MEFs were treated with CHX (100µg/mL) or CHX in combination with MG132 (20µg/mL). Cell lysates were prepared at the 4 h time point, and HA-RAD51D band intensity was normalized to β-actin.