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. Author manuscript; available in PMC: 2017 May 22.
Published in final edited form as: J Mol Biol. 2016 Apr 8;428(10 Pt B):2248–2258. doi: 10.1016/j.jmb.2016.03.032

Figure 1.

Figure 1

Binding of EF-G to vacant ribosomes in the presence of various nucleotides and phosphate analogues (as indicated) measured by the pelleting assay. EF-G was incubated with vacant ribosomes. Half of each sample was pelleted through a sucrose cushion (pellet); the other half was used as a loading control (input). Protein content of ribosome pellets was analyzed using SDS-PAGE. The band corresponding to EF-G and the largest ribosomal protein S1 are indicated by arrows.