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. Author manuscript; available in PMC: 2017 Jun 5.
Published in final edited form as: J Mol Biol. 2016 Apr 15;428(11):2430–2445. doi: 10.1016/j.jmb.2016.04.009

Figure 5. Interactions of RRM2 With RS1 Affect Cytoplasmic-Nuclear Distribution and Dephosphorylation of SRSF1.

Figure 5

A–C) Phosphorylation of 1 μM SRSF1 (A), SRSF13M (B) and RS (C) by SRPK1 (75 nM) in the absence (●) and presence (▲) of PP1 (300 nM). D) Bar graph showing relative phosphorylation levels of SR proteins in the presence of both SRPK1 and PP1. E) Confocal imaging of GFP-SRSF1 and GFP-SRSF13M in HeLa cells. F) Subcellular fractionation of HeLa cells expressing GFP-SRSF1 and GFP-SRSF13M. WT=GFP-SRSF1; Mut=GFP-SRSF13M.