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. Author manuscript; available in PMC: 2017 Jun 17.
Published in final edited form as: Biochem Biophys Res Commun. 2016 May 10;475(1):81–86. doi: 10.1016/j.bbrc.2016.05.040

Fig. 1. GPR30 expression in skeletal muscles of female F344BN rats.

Fig. 1

(A) RT-PCR amplification of a 151-bp GPR30 fragment in soleus muscle. Lane 1, marker; lane 2, soleus muscle; lane 3, negative control (without cDNA). (B) Western blot of soleus muscle showing a single band corresponding to the appropriate molecular weight of GPR30 (~50 kDa). Lane 1, marker; lane 2-3, soleus muscle samples. (C) GPR30 mRNA level, determined by real-time PCR, in extensor digitorum longus (EDL) and gastrocnemius (GAS) of sham-operated and ovariectomized (OVX) female F344BN rats treated with vehicle or G1 for 2 months. Values are mean±SEM; *P<0.05 vs. sham; #P<0.05 vs. OVX-V. n=7/group.