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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: DNA Repair (Amst). 2016 Apr 25;42:44–55. doi: 10.1016/j.dnarep.2016.02.008

Figure 5. Quantitative ssDNA and dsDNA binding data for RAD51 variants and wild-type in the presence and absence of ATP.

Figure 5

Binding of RAD51 protein to AlexaFluor 546-labeled ssDNA and dsDNA homopolymers was monitored by fluorescence quenching as described in Materials and Methods. Blue circles denote wild-type RAD51, red triangles denote Q268P, and green squares denote Q272L. (A) Protein binding to ssDNA in the presence of 1 mM ATP. (B) Protein binding to ssDNA in the absence of ATP. (C) Protein binding to dsDNA in the presence of 1 mM ATP. (D) Protein binding to dsDNA in the absence of ATP. Details of AF546-labeled ssDNA and dsDNA lattices plus other reaction conditions are described in Materials and Methods.