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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: DNA Repair (Amst). 2016 Apr 25;42:44–55. doi: 10.1016/j.dnarep.2016.02.008

Figure 9. DNA strand exchange activity of RAD51 Q272L versus WT with homologous ϕX174 DNA substrates and RPA protein.

Figure 9

Reactions were carried out as described in Materials and Methods and as shown schematically in Figure 8A. (A) Timecourses of reactions containing a total of 7.50 μM RAD51 protein (WT and/or Q272L) at the indicated concentrations. The positions of SS, LDS, NC, and SC (supercoiled ϕX174 DNA) markers (lanes 1–3, 22–24) are indicated next to the gel, along with the position of JM products. Lanes 4–9: Reaction with WT only; Lanes 10–15: Reaction with Q272L only; Lanes 16–21: Reaction with 1:1 mixture of WT and Q272L. (B) Timecourses of reactions containing 3.75 μM WT RAD51 in the absence/presence of equimolar Q272L as indicated. Lanes 1–3, 18–20: Markers. Lanes 4–10: Reaction with WT only; Lanes 11–17: Reaction with mixture of WT and Q272L.