Table 1.
Fusion efficiency with electrofusion (EF) and polyethylene glycol (PEG)
| % of wells with hybridomas @ 1 × 107 splenocytes/plate | % of wells with hybridomas @ 1 × 106 splenocytes/plate | % of wells with hybridomas @ 1 × 105 splenocytes/plate | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Project | PEG | EF | Fold Δ | PEG | EF | Fold Δ | PEG | EF | Fold Δ |
| N372A, B | 75% | 100% | >1 | 16% | 94% | 5.9 | 0% | 18% | NA |
| N378 | 54% | 100% | >1 | 15% | 83% | 5.5 | 2% | 40% | 20.0 |
| L93 | 32% | 86% | 2.7 | 2% | 46% | 23.0 | ND | ND | ND |
| N379 | 51% | 66% | 1.3 | 8% | 24% | 3.0 | 1% | 3% | 3.0 |
| N380 | 96% | 100% | >1 | 14% | 100% | 7.1 | 2% | 66% | 33.0 |
| N376A* | 92% | 100% | >1 | 32% | 74% | 2.3 | 2% | 9% | 4.5 |
NA = not applicable; ND = not done
*
Using larger capacity chamber: 100%, 100% and 50% of wells with hybridomas @ 1 × 107, 1 × 106 and 1 × 105 splenocytes/plate, resulting in >1, >3 and 25.0 fold increases, respectively.