Fig. 2.

The specific interaction between the dimeric 5′-L³⁴⁴ and RHA^NTD occurs independently of NC-binding. (a-b) Secondary structures of 5′-L³⁴⁴ and CES are shown. (c) Dimerization of CES and 5′-L³⁴⁴ were validated on 2% native agarose gel. Lane 1 and 3, indicated transcript dissolved in H₂O and loaded to gel demonstrated mobility of monomer. Lane 2 and 4, indicated transcript incubated with ITC buffer at 37°C overnight demonstrated reduced mobility of dimer. (d) Overlay of ITC isotherms collected for RHA^N300 titrations with 5′-L³⁴⁴ (black) and CES (blue). (e) Superposition of the isotherms collected for RHA^N300 titration with premixed [5′-L³⁴⁴:NC] complex (green) and the titration of RHA^N300 into 5′-L³⁴⁴ (black). (f) Superposition of the isotherms collected for NC titration with premixed [5′-L³⁴⁴: RHA^N300] complex (pink) and the NC titration with 5′-L³⁴⁴ (black).