Table 1.
Primer sequences used for CsCCD2 analysis
| Used for | 5′ → 3′ | |
|---|---|---|
| RACE-5′ and 3′ reactions | F-1 | TGAGTTGGGACCTAGAAGATATG |
| F-2 | TCTGAGGTCAATGTCATCGATG | |
| R-1 | CCACTTCCACTGGTTGGAAATAC | |
| R-2 | CAGGTCCATGAAGATGGCATAG | |
| cDNA amplification | ATG1-F | CTTGACATGGAATCTCCTACTAC |
| ATG2-F | GTATCAATGGCAAATAAGGAG | |
| R | TGTCTCTGCTTGGTGCTTCTGA | |
| Promoter isolation and analysis | Rp1 | GTAGCGGCCCAAATTTACGTGGA |
| Rp2 | TGGAAGGAAGATGGAGGAGCTCT | |
| Rp3 | TGTGGTGTACAAACACCTGCATCC | |
| Rp4 | TTACACGTTAGGCAAGATCGTCCA | |
| Rp5 | CATTTCGGTGGCCCTGTTCGT | |
| Fp | AGGTGATCATAGGTACTCCCTA | |
| gDNA amplification and analysis | F | TCTTGACATGGAATCTCCTACTACT |
| F0 | CAGTAGTATCAATGGCAAATAAGGAG | |
| F1 | CGTCGCAGTGGACTTACTCGA | |
| F2 | AACCCCAAGTTTGCTCCCGTC | |
| F2b | CGTCACGGTTTAAACAAGAAG | |
| F3 | ATACTAGTACTTCGAACATTGA | |
| F4 | TGCCAACCTGGCATCAAATCT | |
| F5 | TGATGAGGATGACGGTTACTTGA | |
| F1-gw-3′ | TCTGAGGTCAATGTCATCGATGCA | |
| F2-gw-3′ | CAGAACCTGTGGCTGTTGTGGAAC | |
| FH | ATGCTTGAATGGAGTGTTTCTGA | |
| F(E + I4) | CGATCCGTTAACTGGTAATTCCT | |
| R(E + I1) | ATCTGCAGGTCGAGTATAGGAGA | |
| R1 | TTGGGGTTAGGGCCAACTCTC | |
| R2 | TAAGCAATCCTCTTAGATCTCCA | |
| R3 | GGAGGCGAGAGGGAATATCCA | |
| R4 | TGTCATGTCTCTGCTTGGTGCT | |
| R5 | TGGAAGCAACCGATACGAGCT | |
| R6 | TGCATCGATGACATTGACCTCA | |
| R7 | TCTCCAATCTGCAGGTCGAGTA | |
| R8 | CCATCTTTAATACGCAATCCATGA | |
| RH | ATCAGGACTCTCAAGGCGACA | |
| For PET28 cloning and activity assays | F-Pet28 | GACGGAGCTCGAATTCTCATGGAATCTCCTACTACT |
| R-Pet28 | TCGCGGATCCGAATTCTCATGTCTCTGCTTGGTGC | |
| cDNA amplification CancyCCD2 | R | CTACGTATTCTTAGAGACTAGT |
| F | TCTGACAGACCATTGTAAACGGA | |
| cDNA amplification CcaCCD2 | R | AGTCGAAGCCCCGTTACTATCT |
| F | GACATGGAATCTCCTACTACTAA |