Skip to main content
. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: J Bioenerg Biomembr. 2016 Mar 1;48(3):325–333. doi: 10.1007/s10863-016-9655-y

Fig. 2.

Fig. 2

Proton translocation rates of two double cysteine substitutions. The reactions were initiated with deamino-NADH (dNADH) to 250 mM final concentration. The fluorescence of ACMA (1 mM) was followed for several minutes. The uncoupler FCCP was added (1 mM) to collapse the generated proton gradient. A. K581C (nuoL) + R99C (nuoK). B. T292C (nuoN) + R99C (nuoK). In each panel the wild type strain is shown for comparison. The traces shown are representative of 2–3 experiments.