Fig. 1.

Inhibition of AKT suppresses the CaP growth: (A) to access the basal expression of pAKT and DAPK3, cell lysates were extracted from a panel of CaP cell lines (PC-3, DU-145, C4-2B, LNCaP, 22Rv1 and C4) and subjected to western blot analysis. Different concentrations of wortmannin (B, D) or LY 294002 (C, E) were used for PC-3 and DU-145 cells and subjected to MTT assays. (F) PC-3 cells were treated with wortmannin and western blot analysis was performed for pAKT, AKT, and DAPK3; actin and GAPDH were used as loading controls.