Fig. 4.
Effect of acute and chronic butyrate treatment on MCT1 and MCT4 protein levels in Caco-2 cells detected with the ProteinSimple WES system. (A) Pherogram view of a representative biological replicate for control and chronic treated butyrate samples analysed for MCT1 and MCT4. (B) Lane view of two representative biological replicates for control and butyrate acute and chronic treated samples analysed for MCT1 and MCT4. (C) Peak areas of MCT1 and MCT4 protein levels normalised to claudin-1, detected in 0.5 mg/ml Caco-2 whole lysate after acute or chronic treatment with 1 mM butyrate and controls treated with vehicle only; n = 6, error bars represent standard error of the mean.