Table 1.
Antibody characterization for immunohistochemistry, ELISA and Western Blot.
| Marker | Cell type | Antigen | Manufacturer, RRID | Concentration | Reference |
|---|---|---|---|---|---|
| BrdU | Dividing cells | Thymidine analog | AbD Serotec Cat# OBT0030 RRID:AB_609568 | 1:400 | Seri et al., 2004 |
| Nestin | Progenitor cells | Intermediate filaments | Millipore Cat# MAB353 RRID:AB_94911 | 1:400 | Seri et al., 2004 |
| Doublecortin | Immature neurons | Contributes to microtubule organization | Millipore Cat# AB2253 RRID:AB_1586992 | 1:500 | Sevc et al., 2014 |
| NeuN | Mature neurons | Nuclear epitope | Merck Cat# MAB377 RRID:AB_11210778 | 1:400 | Seri et al., 2004 |
| TUNEL | Apoptotic cells | Terminal deoxynucleotidyl transferase dUTP nick end | In Situ Cell Death Detection Kit, Fluorescein (Roche Applied Science # 11684795910) | As indicated by kit | Gavrieli et al., 1992 |
| Anti-phospho-IGF-1R | Rabbit. Phosphorylated Tyr1161/Tyr1165/Tyr1166 | Merck Cat# ABE332 RRID:AB_11214503 | 1:200 | Saeed et al., 2007 | |
| Enzyme Immunoassay for Corticosterone | Rabbit. Anti-Corticosterone | Oxford Biomedical Research, EA 66 | As indicated by kit | Arthaud et al., 2015 |