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. 2016 Feb 2;5(2):e285. doi: 10.1038/mtna.2015.55

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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ASCs-miR-27b led to decreased activation of cocultured hepatic stellate cells (HSCs) in vitro. The effects of ASCs-siRNC and ASCs-miR-27b on TGF-β1-activated HSCs were measured in Transwell coculture system. qRT-PCR demonstrated that dose-dependent (a) and time-dependent (b) TGF-β1-induced α-SMA mRNA expression in HSCs was significantly reduced in the presence of ASCs-siRNC, however, was drastically abolished by ASCs-miR-27b. The significant increase of HGF mRNA was observed only in activated HSCs cocultured with ASCs-miR-27b (c). However, no significant difference was found between HSCs cocultured with ASCs-siRNC and medium control. The cells were cultured in six-well culture plates. The data are representative of three independent experiments. All experiments were conducted three times and similar results were observed. The statistics were analyzed with Student's t-test. *P < 0.05; **P < 0.01. ASCs, adipose-derived mesenchymal stem cells; HGF, hepatocyte growth factor; SMA, smooth muscle actin; TGF, transforming growth factor.