Table 2.

Real-time primer sequences and thermocycling conditions.

Target gene	Primer sequence (5′ to 3′)	Thermocycling conditions	Reference/accession number
IL-1β	ACCCCACAAAACATCGGCCAACC	95°C 30 s, 40 cycles of 95°C 5 s, 61.5°C 30 s, and 72°C 30 s	[25]
	TCTTCTCCATTTCCACCCTCTC
IL-10	CGCAGTGCAGAAGAGTCGAC	95°C 30 s, 40 cycles of 95°C 5 s, 61.5°C 30 s, and 72°C 30 s	GU256643
	CCCGCTTGAGATCCTGAAATAT
TNF-α	CTCAACAAGTCTCAGAACAATCAGG	95°C 30 s, 40 cycles of 95°C 5 s, 61.5°C 30 s, and 72°C 30 s	[25]
	TCCTGGTTCCTTCTCCAATCTAGCT
iNOS	GGAGGTACGTCTGCGAGGAGGCT	95°C 30 s, 40 cycles of 95°C 5 s, 61.1°C 30 s, and 72°C 30 s	[8]
	CCAGCGCTGCAAACCTATCATCCA
TGF-β	ACGCTTTATTCCCAACCAAA	95°C 30 s, 40 cycles of 95°C 5 s, 61.5°C 30 s, and 72°C 30 s	AF13694
	GAAATCCTTGCTCTGCCTCA
NF-κB	TATTCAGTGCGTGAAGAAG	95°C 30 s, 40 cycles of 95°C 5 s, 61.5°C 30 s, and 72°C 30 s	LN590704
	TATTAAAGGGGTTGTTCTGT
HSP70	GGCAGAAAGTTTGATGACCCA	95°C 30 s, 40 cycles of 95°C 5 s, 61.5°C 30 s, and 72°C 30 s	[26]
	GCAATCTCCTTCATATTCACC
β-actin	AGACCACCTTCAACTCCATCATG	95°C 30 s, 40 cycles of 95°C 5 s, 61.5°C 30 s, and 72°C 30 s	[25]
	TCCGATCCAGACAGAGTATTTACGC