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. 2016 May 27;5(8):2022–2031. doi: 10.1002/cam4.760

Figure 4.

Figure 4

GABBR1 is critically involved in the proliferation and invasion of HCT116 cells. (A) Baclofen inhibited HCT116 cells proliferation detected by MTT assay. Data shown are means ± SD (n = 4). *P < 0.05, **P < 0.01. (B) BrdU assay showed that baclofen could repress cell proliferation. Data shown are means ± SD (n = 5),*P < 0.05. (C) Baclofen could significantly inhibit HCT116 cells invasion. Data shown are means ± SD (n = 3) **P < 0.01. (D) Inhibition of GABBR1 by two different GABBR1 specific siRNA sequences enhanced HCT116 proliferation detected by MTT assay. Data shown are means ± SD (n = 5). *P < 0.05. (E). BrdU assay showed that siRNA‐mediated inhibition of GABBR1 promoted HCT116 proliferation, which is consistent with MTT assay. Data shown are means ± SD (n = 3),*P < 0.05. (F) siRNA‐mediated inhibition of GABBR1 obviously promoted HCT116 invasion. Right penal showed the statistics of the invasion. Data shown are means ± SD (n = 5),*P < 0.05. BrdU, Bromodeoxyuridine.