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. 2015 Oct 25;7(7):7640–7656. doi: 10.18632/oncotarget.6156

Figure 6. shE6AP mediated E6AP inhibition enhances MNT expression and promotes differentiation in HL60 cells.

Figure 6

a. HL60 cells were transduced with lentiviral shE6AP vector and WCE was prepared post-3 and 4 days of transduction. WCEs were resolved on 10% SDS-PAGE and immunoblotted with anti-MNT, E6AP and β-actin antibodies. b. HL60 cells were transfected with shE6AP as indicated. Cells were washed and labelled with cd11b and its respective IgG-PE conjugated antibody for FACS analysis. c. E6AP knockdown via lentiviral transduction of shE6AP leads to granulocytic differentiation in HL60 cells. After indicated time points, cells were stained with May-Grünwald and Wright's Giemsa Arrows indicate granulocytes/neutrophils. Scale represent 100μm. d. Graphical representation of percentage of G0/G1 cells after knockdown of endogenous E6AP and co-treated with 1μM ATRA post-24h transfection. Data are representative of minimum three independent experiments. Results are given as standard error of mean (+S.E.M.); *P<0.05, **P<0.001, ***P<0.0001. One-way ANOVA with Bonferroni's Multiple Comparison Test was performed using GraphPad Prism Version 5.00.