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. 2015 Dec 22;7(7):7701–7714. doi: 10.18632/oncotarget.6715

Figure 2. Loss of CtIP causes HRR deficiency.

Figure 2

A. Western blot analysis of CtIP in whole cell extracts from MCF7 cells transfected with CtIP or control siRNA (25 nM) for 48 hrs. B. The images of γH2AX foci after 4 Gy IR in control (NC) and CtIP-depleted MCF7 cells at different time points as indicated. Scale bar, 40 μm. C. Quantification of γH2AX foci in Figure 2B. Numbers of γH2AX foci were quantified from triplicated experiments (>50 cells at each condition) and are shown as mean values ± SEM. Statistical significance was calculated by one-way analysis of variance (ANOVA). ( * for P<0.05; ** for P<0.01; where not indicated, the P value was equal or higher than 0.05). D. Wild-type and CtIP-depleted MCF7 cells were irradiated (4 Gy) and fixed 3 hrs later. Rad51 and γH2AX foci were immunodetected with anti-Rad51 and anti-γH2AX antibodies, respectively. Cell nuclei were counterstained with DAPI. Scale bar, 10 μm. E. Quantification of Rad51 foci in Figure 2D. 50 cells at each condition were calculated. Mean ± SEM. Statisitcal significance, ** for P<0.01.