Figure 6. IL-6 up-regulates macrophage M2 phenotype and stimulates IL-4 receptor expression.

A. Human macrophages were cultured in the presence of IL-6 (20 ng/ml) for 24h and the level of Arginase1 and Cd163 measured by Western. Blots were re-probed for GAPDH to confirm equal loading. B. Human macrophages were cultured in the presence of IL-6 (20 ng/ml) for 24h and the level of IL-4 receptor measured by flow cytometry.